Biomarker and Protein Analysis

Explore advanced biomarker and protein analysis techniques, including qPCR, Western blot, ELISA, and Flow-cytometry, to evaluate cancer-specific biomarkers, monitor treatment response, and improve diagnostic accuracy. Analyze tumor biopsies, blood samples, and FFPE tissues for precise research outcomes.

Applications:
  • Evaluate expression levels of cancer-specific biomarkers to identify and validate therapeutic targets.
  • Monitor treatment response by tracking changes in biomarker expression.
  • Conduct multiplex assays to detect multiple biomarkers simultaneously, improving diagnostic accuracy.
Samples:
  • Tumor biopsies.
  • Blood samples.
  • FFPE tissue samples.
  • Primary cancer cells and 3D organoids.
Assays:
  • qPCR (gene in vitro):
  • Quantifies gene expression levels using RNA extracted from cells or tissues.
  • Western blot (protein in vitro):
  • Detects specific proteins and assesses their expression levels using protein extracts from cell or tissue samples.
  • ELISA (protein in vitro or ex vivo):
  • Measures protein concentration in various sample types, including blood and tissue extracts.
  • IHC/IF (protein staining ex vivo):
  • Immunohistochemistry (IHC) and Immunofluorescence (IF) visualize protein expression in tissue sections, cells, and organoids.
  • Flow-cytometry:
  • Analyzes cell surface and intracellular protein expression in single cells from blood, tissue, or cell samples.
  • Luminex® multiplex assay:
  • Simultaneously measures multiple proteins in a single sample using bead-based technology.
Contract research organization, which provides solutions for pharma and diagnostics companies, including consulting, cancer models development, and various assays for drug development.
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