Screen potential anticancer compounds for cytotoxicity to identify promising therapeutic candidates.
Assess cell proliferation rates to understand the effects of treatments on cancer cell growth.
Evaluate apoptosis induction by therapeutic agents to determine their effectiveness.
Monitor metabolic activity as an indicator of cell health and viability.
Samples:
Primary cancer cells and 3D organoids.
Assays:
Cell viability assays (MTT, MTS, CellTiter Glo):
Measure cell viability and metabolic activity using colorimetric or luminescent readouts.
Apoptosis assays (Annexin V, PI staining, Cl Caspase measurement, TUNEL):
Detect and quantify apoptotic cells using flow cytometry, microscopy, or plate-based assays.
High-throughput proliferation assays (MTT, MTS):
Quantify cell proliferation rates using colorimetric assays.
Cell cycle assessment:
Analyzes cell cycle distribution using flow cytometry.
Metabolic assays (CellTiter Glo):
Measure cellular ATP levels as an indicator of metabolic activity.
Luminex® multiplex assay:
Simultaneously measures multiple proteins in a single sample using bead-based technology.
Contract research organization, which provides solutions for pharma and diagnostics companies, including consulting, cancer models development, and various assays for drug development.
